Owen's treatise on Romans 8:13 lays out the Puritan case for the ongoing mortification of sin as the vital work of every believer. Written in 1656 at the height of Puritan pastoral theology, it argues that only the Holy Spirit can accomplish true mortification, and proceeds through fourteen chapters of searching diagnosis and practical direction. Few works have shaped the Reformed understanding of sanctification more deeply across four centuries.

An examination of the nature, occasions, and power of temptation, and the means by which believers can avoid and resist it. Written alongside Mortification of Sin and Indwelling Sin, it forms the second part of Owen's trilogy on the believer's struggle with sin, drawing on extensive scripture to address a subject few Puritan writers treated as thoroughly.

An exposition of Romans 7:21, tracing the law, nature, and workings of sin that remains in the believer after conversion. Owen argues that an accurate understanding of indwelling sin is essential to the Christian's fight against it, completing the trilogy on sin that begins with Mortification of Sin and Of Temptation.